Translational_Unit

Part:BBa_K3523006:Design

Designed by: Kong Yangyang   Group: iGEM20_Xiamen_city   (2020-10-18)


T7 pro-His-katA-His-T7 ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1334
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to meet the type IIS standard, we made a synonymous mutation of Ser283Ser to remove BsaI restriction site.


Source

Catalase (EC:1.11.1.6)

References